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Polimorfisme Gen Growth Hormone dan Hubungannya dengan Sifat Pertumbuhan Sapi Silangan Peranakan Ongole dan Simmental
Muhamad Affan Mu’in, Maria Astuti, Muladno Muladno, Tridjoko Wisnu Murti, Wayan Tunas Artama
Abstract
The present study was conducted to detect polymorphism of growth hormone (GH) gene in Ongole grade
crossed with Simmental bull (SIMPO) and its association with growth traits. Blood samples of 62 cattle were taken from
population of SIMPO cattle were located in the sub-province region of Bantul, Special Region of Yogyakarta. A 211 bp
fragment of GH gene spanning from the forth intron region (49 bp) to fifth of exon (162 bp) was amplified and digested
with AluI restriction enzyme to identified polymorphism at this locus. The resulted indicated that two genotypes LL and LV
were found at the GH gene in SIMPO population cattle. The frequencies of L and V alleles were 0.82 and 0.18,
respectively. In SIMPO calves, the average birth weight, 2 months body weight and daily body weight gains of LV
genotypes were tend to higher than that of LL genotypes.
crossed with Simmental bull (SIMPO) and its association with growth traits. Blood samples of 62 cattle were taken from
population of SIMPO cattle were located in the sub-province region of Bantul, Special Region of Yogyakarta. A 211 bp
fragment of GH gene spanning from the forth intron region (49 bp) to fifth of exon (162 bp) was amplified and digested
with AluI restriction enzyme to identified polymorphism at this locus. The resulted indicated that two genotypes LL and LV
were found at the GH gene in SIMPO population cattle. The frequencies of L and V alleles were 0.82 and 0.18,
respectively. In SIMPO calves, the average birth weight, 2 months body weight and daily body weight gains of LV
genotypes were tend to higher than that of LL genotypes.
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