Bakteri Asam Laktat dari Intestin Ayam sebagai Agensia Probiotik
Abstract
Lactic acid bacteria have been suggested to have several beneficial effects on human and animals. These
bacteria, indigenous to the gastro-intestinal tract, are important in regulating the balance among the desirable and
undesirable intestinal microflora and in controlling enteric pathogenic infection in the host. Objectives of this research are
to obtain lactic acid bacteria isolates from gastro-intestinal tract of chicken and to screen their ability as a probiotic agent
i.e., their antagonistic against pathogenic bacteria, their survival at low pH and high concentration of bile salt. In this
research, 74 samples used as sources of bacteria, and among them only 11 samples could be isolated as lactic acid bacteria
with the total number of isolates of 61. Based on the preliminary screening i.e., their antagonistic factor against
pathogenic bacteria, 20 isolates was further studied. Based on the identification scheme, these isolates belong to three
species, i.e., Lactobacillus murinus, Pediococcus acidilactici, and Streptococcus thermophilus. The result showed that
most isolates grow well in the media with the initial pH of 5.5, but their growth were retarded when the initial pH 3.5.
Only one isolate Streptococcus thermophilus Kp-2 showed its growth at initial pH of 3.5. All isolates did not show any
growth at initial pH 2.5, though their viability still high. The result based on the isolates resistance to bile salt showed that
most isolates could grow at media with 0.20% of bile salt. Their growth was inhibited with the increasing bile salt
concentration. However, few isolates could grow well at media with 1% of bile salt. Based on their characteristics three
isolates i.e., Lactobacillus murinus Ar-3, Streptococcus thermophilus Kp-2, and Pediococcus acidilactici Kd-6 were
selected as probiotic agents for the continuing research. i.e. production of biomass and its application to chicken production
bacteria, indigenous to the gastro-intestinal tract, are important in regulating the balance among the desirable and
undesirable intestinal microflora and in controlling enteric pathogenic infection in the host. Objectives of this research are
to obtain lactic acid bacteria isolates from gastro-intestinal tract of chicken and to screen their ability as a probiotic agent
i.e., their antagonistic against pathogenic bacteria, their survival at low pH and high concentration of bile salt. In this
research, 74 samples used as sources of bacteria, and among them only 11 samples could be isolated as lactic acid bacteria
with the total number of isolates of 61. Based on the preliminary screening i.e., their antagonistic factor against
pathogenic bacteria, 20 isolates was further studied. Based on the identification scheme, these isolates belong to three
species, i.e., Lactobacillus murinus, Pediococcus acidilactici, and Streptococcus thermophilus. The result showed that
most isolates grow well in the media with the initial pH of 5.5, but their growth were retarded when the initial pH 3.5.
Only one isolate Streptococcus thermophilus Kp-2 showed its growth at initial pH of 3.5. All isolates did not show any
growth at initial pH 2.5, though their viability still high. The result based on the isolates resistance to bile salt showed that
most isolates could grow at media with 0.20% of bile salt. Their growth was inhibited with the increasing bile salt
concentration. However, few isolates could grow well at media with 1% of bile salt. Based on their characteristics three
isolates i.e., Lactobacillus murinus Ar-3, Streptococcus thermophilus Kp-2, and Pediococcus acidilactici Kd-6 were
selected as probiotic agents for the continuing research. i.e. production of biomass and its application to chicken production
Keywords
acid bacteria, gastro- intestinal tract of chicken, probiotic agents