Ekstraksi Kolagen Cakar Ayam dengan Berbagai Jenis Larutan dan Lama Perendaman
Abstract
The objective of this research was to know the ability of various acidic solutions on dissolving collagen
chicken feet, with different soaked time. Each acid 5 percent (v/v), collagen extraction was done by washing chicken feet
and then cutted into small pieces and finally grinded. Every 100 gram treatment was soaked in acetic acid (a1), citric acid
(a2), lactic acid (a3) and hydrochloric acid (a4), for 12, 24 and 36 hours. Precipitated collagen in the filtrate was 5 percent
NaOH to reach the neutral pH (pH 7). Collagen precipitate was separated by filtration usingfilter paper and then
rendement was calculated, HPLC was used to determin amino acid composition, and SDS-PAGE was use determin the type
of collagen. This experiment use factorial completely randomized design (CRD) 4 x 3 and three time replication. Result
showed that lactic acid has highest capability to dissolve collagen, while citric acid the lowest. Combination of acid
solution and soaking time had significant (P
chicken feet, with different soaked time. Each acid 5 percent (v/v), collagen extraction was done by washing chicken feet
and then cutted into small pieces and finally grinded. Every 100 gram treatment was soaked in acetic acid (a1), citric acid
(a2), lactic acid (a3) and hydrochloric acid (a4), for 12, 24 and 36 hours. Precipitated collagen in the filtrate was 5 percent
NaOH to reach the neutral pH (pH 7). Collagen precipitate was separated by filtration usingfilter paper and then
rendement was calculated, HPLC was used to determin amino acid composition, and SDS-PAGE was use determin the type
of collagen. This experiment use factorial completely randomized design (CRD) 4 x 3 and three time replication. Result
showed that lactic acid has highest capability to dissolve collagen, while citric acid the lowest. Combination of acid
solution and soaking time had significant (P
Keywords
Chicken feet, acids, soaking time, collagen